Engineering proteins with tailored nanomechanical properties: a single molecule approach.
نویسنده
چکیده
Elastomeric proteins underlie the elasticity of natural adhesives, cell adhesion and muscle proteins. They also serve as structural materials with superb mechanical properties. Single molecule force spectroscopy has made it possible to directly probe the mechanical properties of elastomeric proteins at the single molecule level and revealed insights into the molecular design principles of elastomeric proteins. Combining single molecule atomic force microscopy and protein engineering techniques, it has become possible to engineer proteins with tailored nanomechanical properties. These efforts are paving the way to design artificial elastomeric proteins with well-defined nanomechanical properties for application in nanomechanics and materials sciences.
منابع مشابه
Single molecule force spectroscopy reveals engineered metal chelation is a general approach to enhance mechanical stability of proteins.
Significant mechanical stability is an essential feature shared by many elastomeric proteins, which function as molecular springs in a wide variety of biological machinery and biomaterials of superb mechanical properties. Despite the progress in understanding molecular determinants of mechanical stability, it remains challenging to rationally enhance the mechanical stability of proteins. Using ...
متن کاملRecombination of protein fragments: a promising approach toward engineering proteins with novel nanomechanical properties.
Combining single molecule atomic force microscopy (AFM) and protein engineering techniques, here we demonstrate that we can use recombination-based techniques to engineer novel elastomeric proteins by recombining protein fragments from structurally homologous parent proteins. Using I27 and I32 domains from the muscle protein titin as parent template proteins, we systematically shuffled the seco...
متن کاملDomain insertion effectively regulates the mechanical unfolding hierarchy of elastomeric proteins: toward engineering multifunctional elastomeric proteins.
The architecture of elastomeric proteins controls fine-tuned nanomechanical properties of this class of proteins. Most elastomeric proteins are tandem modular in structure, consisting of many individually folded domains of varying stability. Upon stretching, these elements unfold sequentially following a strict hierarchical pattern determined by their mechanical stability, where the weakest ele...
متن کاملDNA origami-based shape IDs for single-molecule nanomechanical genotyping
Variations on DNA sequences profoundly affect how we develop diseases and respond to pathogens and drugs. Atomic force microscopy (AFM) provides a nanomechanical imaging approach for genetic analysis with nanometre resolution. However, unlike fluorescence imaging that has wavelength-specific fluorophores, the lack of shape-specific labels largely hampers widespread applications of AFM imaging. ...
متن کاملEngineering proteins with novel mechanical properties by recombination of protein fragments.
The mechanical properties of proteins are crucial in living cells as proteins serve as basic units in cells to constantly sense, generate, and bear mechanical forces. Outside the cell, nature exploits the mechanical features of proteins and produces a variety of materials with superb mechanical properties (e.g. spider dragline silk) which often outperform man-made materials. Studies of the mech...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Organic & biomolecular chemistry
دوره 5 21 شماره
صفحات -
تاریخ انتشار 2007